Simbiozna učinkovitost autohtonih sojeva kvržičnih bakterija koje noduliraju grah

master's thesis

• Author: Mihaela Rak
• Mentor: Sanja Sikora (mentor)
• Committee member: Sanja Sikora (predsjednik povjerenstva)
• Committee member: Nina Toth (član povjerenstva)
• Committee member: Mirjana Herak Ćustić (član povjerenstva)
• Committee member: Ivana Rajnović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Microbiology); Zagreb
• Defense date and country: 2016-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Selekcija visoko učinkovitih sojeva simbioznih fiksatora dušika od presudne je
važnosti za uspjeh predsjetvene bakterizacije graha jer se sojevi kvržičnih bakterija značajno
razlikuju po svojoj učinkovitosti, kompetitivnosti i kompatibilnosti. Cilj ovog istraživanja je
odrediti nodulacijsku sposobnost autohtonih sojeva kvržičnih bakterija koje noduliraju grah,
procijeniti njihovu simbioznu učinkovitost i kompatibilnost sa dvije sorte graha. U svrhu
istraživanja postavljen je dvofaktorijelni vegetacijski pokus u plasteniku. Faktori pokusa su
bili sojevi Rhizobium spp. (12 autohtonih sojeva izoliranih sa područja sjeverozapadne
Hrvatske, jedan referentni soj i nebakterizirana kontrola) i dvije sorte graha (Trešnjevac i
Slavonski zeleni). U fazi pune cvatnje određivana je masa suhe tvari kvržica, masa suhe tvari
biljke te sadržaj ukupnog dušika u nadzemnom dijelu biljke. Istraživanjem su utvrđene
signifikantne razlike za sva istraživana svojstva ovisno o primijenjenom soju kvržičnih
bakterija kao i sorti graha. Utvrđena je kompatibilnost autohtonih i referentnih sojeva s
ispitivanim sortama graha. Sorta Trešnjevac bila je signifikantno bolja u odnosu na Slavonski
zeleni po svim mjerenim parametrima. Na temelju provedenog istraživanja utvrđeno je da se
autohtoni sojevi razlikuju u svojoj simbioznoj učinkovitosti. Rezultati istraživanja pokazuju
da je najveća masa suhe tvari kvržica utvrđena uz primjenu autohtonog soja 12. Signifikantno
najveća masa suhe tvari biljke ostvarena je uz primjenu autohtonih sojeva 1 i 7, dok je najveći
sadržaj ukupnog dušika u nadzemnom dijelu biljke ostvaren uz primjenu autohtonog soja 7.
Dobiveni rezultati ukazuju da se navedeni autohtoni sojevi odlikuju najvećom simbioznom
učinkovitošću i da bi mogli poslužiti u daljnjim istraživanjima za proizvodnju preparata za
predsjetvenu bakterizaciju graha na ovom području.

Abstract (english)

Selection of highly efficient nitrogen fixing bacteria is very important for successful
legume inoculation due to the fact that the strains of nodule bacteria strongly differ in their
symbiotic efficiency, competitiveness and compatibility. The main aim of this study was to
determine the nodulation ability of indigenous common bean rhizobia, to evaluate their
symbiotic efficiency and to determine compatibility with two cultivars of common bean. For
the purpose of this research, twofactorial vegetation trial in a greenhouse was set up. Trial
factors were strains of Rhizobium spp. (12 indigenous strains isolated from northwest Croatia,
reference strain and non inoculated control) and common bean cultivars (Trešnjevac and
Slavonian green). In the full blossom phase, dry weight of nodules, dry weight of plants and
nitrogen content in plants was determined. The study showed significant differences for all
studied characteristics depending on the selected strains of rhizobia and cultivars of common
bean. The compatibility of indigenous and reference strains with tested common bean
cultivars was determined. The cultivar Trešnjevac was significantly better than Slavonian
green in all measured parameters. The results revealed that the indigenous common bean
rhizobial strains considerable differ in their symbiotic efficiency. The highest values for dry
weight of nodule were obtained by application of indigenous strain 12. Significantly higher
dry weight of plant was determined with the use of strains 1 and 7 while the highest nitrogen
content in plants was determined by application of indigenous strain 7. The obtained results
suggest that these indigenous strains can be characterized as the most efficient ones and could
be used in further research oriented to the improvement of inoculant quality.

• Keywords: simbiozna fiksacija dušika; grah; autohtoni sojevi; Rhizobium spp.
• Keywords (english): symbiotic nitrogen fixation; common bean; indigenous strains; Rhizobium spp.
• Language: croatian
• URN:NBN: urn:nbn:hr:204:756845
• Study programme: Title: Agroecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologija (magistar/ magistra inženjer/inženjerka agroekologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Repository: Repository Faculty of Agriculture University of Zagreb
• Created on: 2016-12-01 07:28:40