Povezanost polimorfizma Val 108/158Met katehol-o-metil transferaze i kliničkih simptoma posttraumatskog stresnog poremećaja

master's thesis

• Title (english): Association of catehol-o-methyltransferase Val108/158Met polymorphism and clinical symptoms of posttraumatic stress disorder
• Author: Nikolina Medaković
• Mentor: Gordana Nedić Erjavec (mentor)
• Mentor: Maja Matulić (mentor)
• Committee member: Maja Matulić (predsjednik povjerenstva)
• Committee member: Nada Oršolić (član povjerenstva)
• Committee member: Martina Šeruga Musić (član povjerenstva)
• Committee member: Sofia Ana Blažević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2020-01-22, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Posttraumatski stresni poremećaj (PTSP) je stresom izazvan poremećaj koji se razvija nakon izlaganja traumatskom događaju. Patofiziologija PTSP-a uključuje poremećaje dopaminergičke funkcije. Funkcionalni polimorfizam rs4680 (Val108/158Met) katehol-o-metil transferaze (COMT) je zbog utjecaja na aktivnost samog enzima, važan za regulaciju degradacije dopamina, posebice u prefrontalnom korteksu. Oblik enzima s valinom se povezuje s njegovom povećanom aktivnošću. Cilj ovog diplomskog rada bilo je ispitati povezanost genotipa obzirom na polimorfizam rs4680 gena COMT i težine simptoma PTSP-a procijenjenih kliničkom ljestvicom pozitivnih i negativnih simptoma (PANSS). Studija je uključila 573 muškarca oboljelih od PTSP-a izazavnog ratnim iskustvom kod kojih je težina simptoma prema PANSS-u ovisila o tome jesu li pušači. Istraživanje je pokazalo značajnu povezanost genotipa Met/Met s manjim brojem bodova ostvarenih na PANSS podljestvicama za kogniciju, psihotične simptome i agitaciju kod nepušača te s većim brojem bodova ostvarenih na općoj psihopatološkoj ljestvici kod pušača. Zaključno, polimorfizam rs4680 gena COMT je povezan s težinom pojedinih simptoma procjenjenih prema PANSS-u.

Abstract (english)

Posttraumatic Stress Disorder (PTSD) is a stress-related disorder caused by an exposure to a traumatic event. Pathophysiology of PTSD involves dysfunctions in dopaminergic system. A functional polymorphism rs4680 (Val108/158Met) of catechol-o-methyltransferase (COMT), due to its effect on the activity of the enzyme, plays an important role in dopamine degradation, especially in prefrontal cortex. The valine variant is associated with increased enzymatic activity. The aim of this thesis was to examine the association of COMT rs4680 genotypes and severity of PTSD symptoms according to positive and negative syndrome scale (PANSS). The study included 573 men with combat related PTSD, whose symptoms' severity according to the PANSS was related to their smoking status. Results indicated a significant association of Met/Met genotype with lower scores on PANSS cognitive, psychosis and excitement subscales in nonsmokers and with higher scores on general psychopathology scale in smokers. In conclusion, the COMT rs4680 polymorphism contributes to the severity of individual PTSD symptoms assessed using the PANSS.

• Keywords: posttraumatski stresni poremećaj; katehol-o-metil transferaza; rs4680; polimorfizam; ljestvica pozitivnih i negativnih simptoma
• Keywords (english): posttraumatic stress disorder; catechol-o-methyltransferase; rs4680; polymorphism; positive and negative syndrome scale
• Language: croatian
• URN:NBN: urn:nbn:hr:217:714505
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Repository: Repository of the Faculty of Science
• Created on: 2020-03-16 10:04:38