Insurance companies are major institutional investors on financial markets. Although it does not hold a large share of the financial system of the Republic of Croatia and they have a growing trend, their insurance indicators don’t have positive trends and they are falling with gross premiums and net income. Possibility of classification of financial investments in the various categories of financial assets provides great possibilities in terms of selection attractive management strategy portfolio. In this context, insurance companies invest assets for covering technical and mathematical reserves. This paper presents the effects of subsequent measurement of financial assets in the regression analysis with profitability and certain indicators of profitability. It has shown that the effects of subsequent measurement of financial assets have very little impact on the profitability and profitability indicators of insurance companies.