Bakterije mliječne kiseline izolirane iz majčinog mlijeka

undergraduate thesis

• Title (english): Lactic acid bacteria isolated from breast milk
• Author: Ana Širić
• Mentor: Blaženka Kos (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2018-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Majčino mlijeko sadrži brojne supstance, a uz to i žive bakterije koje koloniziraju neonatalni gastrointestinalni trakt (GIT) dojenčeta. Cilj ovog rada bio je izolirati bakterije mliječne kiseline (BMK) iz majčinog mlijeka. Izolacija je provedena uspješno, pri čemu je izolirano 60 novih bakterijskih sojeva kojima su ispitani genotipski profili bakterijskih izolata amplifikacijom DNA korištenjem univerzalne M13 (5’-GAGGGTGGCGGTTCT-3’) početnice. Ispitana je također prisutnost gena koji kodiraju za S-proteine u genomu sojeva izoliranih iz majčinog mlijeka. RAPD-PCR analiza produkata dobivenih sa specifičnim Usl početnicama je pokazala da svi ispitani sojevi BMK u genomu sadrže jedan ili više gena koji kodiraju za S-proteine. Rezultati SDS-PAGE pokazuju da nijedan uzorak površinskih proteina ne sadrži karakterističnu proteinsku vrpcu koja bi ukazala da ispitivani sojevi BMK eksprimiraju S-proteine.

Abstract (english)

Breast milk contains numerous substances and also alive bacteria colonizing neonatal gastrointestinal tract (GIT) of an infant. Purpose of this thesis was to isolate lactic acid bacteria (LAB) from breast milk. Isolation was carried out successfully, whereby 60 new bacterial strains were isolated and examined for genotypic profiles with DNA amplification using universal M13 (5’-GAGGGTGGCGGTTCT-3’) primer. Existence of genes which codes for S-proteins in genome of strains isolated from breast milk was also examined. RAPD-PCR analysis of products obtained with specific Usl primers showed that all tested LAB strains contain one or more genes encoding S-proteins in the genomes. Results indicate that neither sample of surface proteins does not contain characteristical protein band, which represents expression of S-proteins in examined LAB strains.

• Keywords: bakterije mliječne kiseline; gastrointestinalni trakt; majčino mlijeko; S-proteini
• Keywords (english): breast milk; gastrointestinal tract; lactic acid bacteria; S-proteins
• Language: croatian
• URN:NBN: urn:nbn:hr:159:493257
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Repository: Repository of the Faculty of Food Technology and Biotechnology
• Created on: 2018-10-04 09:14:23