Ispitivanje citotoksičnosti prirodnih niskotemperaturnih eutektičkih otapala za farmaceutsku primjenu

undergraduate thesis

• Title (english): Evaluation of cytotoxicity of deep eutectic solvents for pharmaceutical application
• Author: Sunčica Šarac
• Mentor: Manuela Panić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2021-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Niskotemperaturna eutektička otapala su otapala sastavljena od dvije ili više komponenata od kojih jedna djeluje kao donor, a druga kao akceptor vodikove veze. Povezivanjem se dobije smjesa čija je temperatura tališta znatno niža od one pojedinih komponenata. Niskotemperaturna eutektička otapala smatraju se netoksičnima, ali je prije primjene u industriji potrebno ispitati njihov utjecaj na ljude i okoliš. U ovom radu ispitan je utjecaj triju niskotemperaturnih eutektičkih otapala (timol:kumarin, timol:dekanska kiselina i timol:oktanska kiselina) i terapeutskog eutektičkog otapala (timol:oktanska kiselina:klotrimazol) na HaCaT i HeLa staničnu liniju MTS metodom. Otapala su pokazala nizak inhibitoran učinak na rast stanica te se mogu smatrati nisko citotoksičnim otapalima.

Abstract (english)

Deep eutectic solvents (DESs) are composed of two or more components, one of them being hydrogen bond donor and the other one acceptor. The melting point of the eutectic mixture is significantly lower than that of either of the components. DESs are considered to be non-toxic. However, prior to their large-scale use, it is necessary to determine their impact on human and the environment. In this bachelor thesis, the impact of three DESs (Ty:Cou, Ty:C10, Ty:C8) and one THEDES (Ty:C8:CLO) on HaCaT and HeLa cell line was examined using MTS method. Solvents showed low inhibitory effect on cell growth and can be considered low-cytotoxic.

• Keywords: citotoksičnost; eutektička otapala; HaCaT stanična linija; klotrimazol
• Keywords (english): cytotoxicity; clotrimazole; deep eutectic solvents; HaCaT cell line
• Language: croatian
• URN:NBN: urn:nbn:hr:159:029137
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Repository: Repository of the Faculty of Food Technology and Biotechnology
• Created on: 2021-07-28 11:37:29