Rast CHO stanica u bazalnom hranjivom mediju s proteinskim hidrolizatom sjemenki lana i albuminom

undergraduate thesis

• Title (english): CHO cell growth in basal nutrient medium with flaxseed protein hydrolysate and albumin
• Author: Mia Galić
• Mentor: Igor Slivac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2022-06-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu obrađena je tema uzgoja adherentnih stanica ovarija kineskog hrčka CHO DP- 12 u bazalnom hranjivom mediju (DMEM) sa sljedećim dodacima: 10% fetalnog goveđeg seruma (standard), s dodatkom albumina i hidrolizata proteina uljne pogače lana te s dodatkom samog albumina. Albumin je glavni protein u fetalnom goveđem serumu te se ispituje njegov značaj za rast i održavanje stanica, dok se biljni proteinski hidrolizati često dodaju kao zamjena proteinima životinjskog podrijetla. Krajnji cilj ovakvih ispitivanja jest pronaći optimalan medij za uzgoj stanica sa što manjom količinom dodanog seruma, budući da postoji mnogo problema vezanih za upotrebu seruma kao što su cijena, sigurnost, potencijalne kontaminacije te etička pitanja vezana za proizvodnju seruma. Eksperiment je proveden na način da su stanice uzgajane u navedenim medijima te je određenih dana
bojilom Trypan plavo praćen rast stanica. Rezultati pokazuju da je najveća koncentracija i najbrži rast u mediju koji sadrži 10% fetalnog goveđeg seruma, a najmanja koncentracija u mediju koji je sadržavao samo albumin. Albumin sa ili bez dodanih lanenih hidrolizata nije jednakovrijedna zamjena za fetalni serum.

Abstract (english)

The subject of this thesis is culturing adherent chinese hamster ovary cell line CHO DP-12 in basal culture media (DMEM) containing different supplements: 10% fetal bovine serum (standard), with different concentrations of albumin and protein hydrolysates and with the addition of albumin itself. Albumin is the major protein in fetal bovine serum and its effect on cell growth and maintenance is examined in order to find the optimal medium for culturing serum-free cells, as there are many problems regarding serum use such as price, safety, potential contamination, and ethical issues related to serum production. The experiment was carried out in such a way that the cells were grown in the previously mentioned media, and on certain days the cells were counted using the Trypan blue method, thus monitoring their growth and development. The results show that the highest concentration and the fastest growth is in the medium containing 10% of fetal bovine serum, and the lowest concentration in the medium containing only albumin. Supplementation of DMEM with albumin and/or flaxseed hydrolysates cannot is not recommended as serum replacement.

• Keywords: CHO DP-12; albumin; hranjivi medij; serum; proteinski hidrolizati uljne pogače lana
• Keywords (english): CHO DP-12; albumin; cell culture medium; serum; flaxseed protein hydrolysate
• Language: croatian
• URN:NBN: urn:nbn:hr:159:432717
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Repository: Repository of the Faculty of Food Technology and Biotechnology
• Created on: 2024-04-10 12:08:09