Antioksidacijski kapacitet tkiva češljače Aequipecten opercularis

master's thesis

• Title (english): Antioxidant capacity of the queen scallop Aequipecten opercularis tissue
• Author: Anamarija Prgić
• Mentor: Ines Kovačić (mentor)
• Mentor: Petar Kružić (komentor)
• Committee member: Petar Kružić (predsjednik povjerenstva)
• Committee member: Sven Jelaska (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Ivana Buj (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-09-21, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Antioksidansi su važne molekule koje inhibiraju djelovanje nestabilnih slobodnih radikala u
stanici donirajući im jedan svoj elektron. U novije vrijeme sve je veća potreba za prirodnijim
antioksidansima odnosno bioaktivnim tvarima. Jedni od spojeva koji su pokazali najveći
potencijal za antioksidacijsku zaštitu organizma od slobodnih radikala su fenolni spojevi.
Istraživanja fenolnih spojeva se baziraju uglavnom na biljkama. Istraživanja za prirodnijim
antioksidansima iz morskih organizama su u porastu, no i dalje je vrlo mali broj dostupnih
podataka o antioksidacijskom potencijalu morskih školjkaša. Stoga se ovaj diplomski rad bavi
određivanjem antioksidativne sposobnosti češljače Aequipecten opercularis pomoću triju
spektrofotometrijskih metoda (ABTS, FRAP, DPPH). Za ekstrakciju fenolnih spojeva su
korištena tri različita otapala deionizirana voda (dH2O), 70 % etanol (EtOH), te 90 % metanol
(MeOH). Jedinke su uzorkovane na području Medulinskog zaljeva u rano proljeće. Kako bi
se prikupili podaci za morfometrijska mjerenja i određivanje indeksa kondcije nad jedinkama
su provedena morfometrijska mjerenja i vaganja. Na temelju dobivenih podataka je određen
antioksidacijski kapacitet tkiva češljače. Antioksidacijski kapacitet bio je najvećih vrijednosti
kod vodenog ekstrakta u sve tri korištene metode. Metoda ABTS je pokazala najbolje
rezultate antioksidacijskog kapaciteta, zatim slijedi DPPH metoda i na kraju FRAP metoda.
Fenolni spojevi u tkivu češljače Aequipecten opercularis pokazuju antioksidacijski potencijal,
te bi se u budućnosti mogli koristiti za prehranu čovjeka, a u svrhu obrane organizma od
slobodnih radikala i sprečavanja ili ublažavanja bolesti koje oni izazivaju.

Abstract (english)

Antioxidants are important molecules that inhibit the action of unstable free radicals in the
cell by donating one of its electrons. In recent times, there is an increasing need for natural
antioxidants, ie bioactive substances. One of the compounds that showed the greatest potential
for antioxidant protection of the body against free radicals are phenolic compounds. Research
on phenolic compounds is mainly based on plants. Research on natural antioxidants from
marine organisms is on the rise, but there is still very little available data on the antioxidant
potential of marine shellfish. Therefore, this thesis deals with the determination of the
antioxidant abilities of the comb Aequipecten opercularis using three spectrophotometric
methods (ABTS, FRAP, DPPH). For the extraction of phenolic compounds, three different
solvents were used: deionized water (dH2O), 70% ethanol (EtOH), and 90% methanol
(MeOH). Individuals were sampled in the Medulin Bay area in early spring. In order to collect
data for morphometric measurements and determination of the condition index, morphometric
measurements and weighing were performed on the individuals. Based on the obtained data,
the antioxidant capacity of comb tissue was determined. The antioxidant capacity was the
highest in the aqueous extract in all three methods used. The ABTS method showed the best
results of antioxidant capacity, followed by the DPPH method and finally the FRAP method.
Phenolic compounds in the tissue of the comb Aequipecten opercularis show antioxidant
potential, and in the future could be used for human use in order to defend the body against
free radicals and prevent or alleviate the diseases they cause.

• Keywords: slobodni radikali; antioksidacijski kapacitet; fenoli; školjkaši
• Keywords (english): free radicals; antioxidant potential; phenols; bivalves
• Language: croatian
• URN:NBN: urn:nbn:hr:217:918021
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Repository: Repository of the Faculty of Science
• Created on: 2022-10-21 10:57:35