IDENTIFIKACIJA I BIOLOŠKA AKTIVNOST SLOBODNIH HLAPLJIVIH SPOJEVA RODA Veronica L. (PLANTAGINACEAE)

doctoral thesis

• Title (english): IDENTIFICATION AND BIOLOGICAL ACTIVITY OF FREE VOLATILE COMPOUNDS OF THE GENUS Veronica L. (PLANTAGINACEAE)
• Author: Marija Nazlić
• Mentor: Valerija Dunkić (mentor)
• Committee member: Željka Vidaković-Cifrek (predsjednik povjerenstva)
• Committee member: Zlatko Liber (član povjerenstva)
• Committee member: Martina Grdiša (član povjerenstva)
• Committee member: Ivica Šamanić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Odabrane vrste roda Veronica (čestoslavice, porodica Plantaginaceae) - V. saturejoides ssp.
saturejoides, V. austriaca ssp. jacquinii i V. officinalis, prikupljene su na prirodnim staništima
u Republici Hrvatskoj. Za ove vrste istražen je fitokemijski sastav slobodnih hlapljivih spojeva
eteričnih ulja i hidrosola plinskom kromatografijom-masenom spektrometrijom. Prevladavajući
spojevi bili su heksahidrofarnezil aceton i heksadekanska kiselina. Iste vrste testirane su na
antioksidativno, antiproliferativno i antifitovirusno djelovanje. Vrsta V. saturejoides ssp.
saturejoides i V. officinalis pokazale su jača djelovanja od vrste V. austriaca ssp. jacquinii u
većini testova. Mikromorfološkim istraživanjem u sve tri vrste identificirani su žljezdani
trihomi u kojima se primarno događa sinteza istraživanih spojeva. U drugom dijelu doktorskog
rada genetički su analizirane ITS regije i slobodni hlapljivi spojevi za 18 vrsta čestoslavica. Uz
gore navedene vrste materijal je prikupljen i analiziran za: V. longifolia, V. acinifolia, V.
anagallis-aquatica, V. beccabunga, V. catenata, V. serpyllifolia, V. anagalloides, V. montana,
V. arvensis, V. chamaedrys, V. dalmatica, V. persica, V. polita, V. cymbalaria i V. hederifolia.
Usporedbom klastera utvrđenim genetskim istraživanjima s fitokemijskom raspodjelom
različitih hlapljivih tvari, donesen je zaključak o mogućnosti korištenja određenih hlapljivih
komponenti kao kemofenetskih markera za rod Veronica.

Abstract (english)

Selected species of the genus Veronica (speedwells, family Plantaginaceae) - V. saturejoides
ssp. saturejoides, V. austriaca ssp. jacquinii and V. officinalis, were collected in natural habitats
in the Republic of Croatia. For these species, the phytochemical composition of free volatile
compounds of essential oils and hydrosols was investigated by gas chromatography-mass
spectrometry. The predominant compounds were hexahydrofarnesyl acetone and hexadecanoic
acid. The same species were tested for antioxidant, antiproliferative and antiphytoviral activity.
The species V. saturejoides ssp. saturejoides and V. officinalis showed stronger effects than V.
austriaca ssp. jacquinii in most tests. Micromorphological research in all three species
identified the glandular trichomes in which the synthesis of the investigated compounds
primarily occurs. In the second part of the dissertation, the ITS regions and free volatile
compounds of 18 species of speedwells were genetically analyzed. In addition to the above
mentioned species, the material was collected and analyzed for: V. longifolia, V. acinifolia, V.
anagallis-aquatica, V. beccabunga, V. catenata, V. serpyllifolia, V. anagalloides, V. montana,
V. arvensis, V. chamaedrys, V. dalmatica, V. persica, V. polita, V. cymbalaria and V.
hederifolia. By comparing the clusters obtained from genetic research with the phytochemical
distribution of various volatile substances, a conclusion was reached about the possibility of
using certain volatile components as chemophenetic markers for the genus Veronica.

• Keywords: čestoslavice; Veronica; slobodni hlapljivi spojevi; kemofenetski markeri; biološka aktivnost
• Keywords (english): speedwells; Veronica; free volatile compounds; chemophenetic markers; biological activity
• Language: croatian
• URN:NBN: urn:nbn:hr:217:996836
• Promotion: 2023
• Project: Number: IP-2020-02-8425; Title: Hrvatske vrste roda Veronica: fitotaksonomija i biološka aktivnost; Title: Croatian Veronica species: Phytotaxonomy and Biological Activity; Acronym: CROVeS-PhyBA; Leader: Valerija Dunkić; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Repository: Repository of the Faculty of Science
• Created on: 2023-10-02 13:14:29