Imunohistokemijska izraženost E-kadherina, N-kadherina i beta-katenina u onkocitomu, kromofobnom karcinomu i karcinomu svijetlih stanica bubrega

doctoral thesis

• Title (english): Immunohistochemical expression of E-cadherin, N-cadherin and beta-catenin in oncocytoma, chromophobe and clear cell renal carcinoma
• Author: Marinko Vučić
• Mentor: Davor Tomas (mentor); MBZ: 270320
• Committee member: Inga Marijanović (član povjerenstva)
• Committee member: Davor Tomas (član povjerenstva); MBZ: 270320
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Novija istraživanja su pokazala da je izraženost staničnih adhezivnih molekula u nekim tumorima od presudne važnosti u razvoju tumorske invazije. Hipoteza ovog istraživanja bila je da postoje razlike u imunohistokemijskoj izraženosti E-kadherina, N-kadherina i beta-katenina između onkocitoma, karcinoma bubrega kromofobnog tipa i karcinoma bubrega svijetlih stanica. Cilj ovog istraživanja bio je imunohistokemijski odrediti i usporediti izraženost E-kadherina, N-kadherina i beta-katenina u tkivu bubrega, onkocitomu i karcinomu bubrežnih stanica (kromofobnom i tipu svijetlih stanica). U istraživanju se koristio arhivski materijal parafinskih blokova 29 onkocitoma, 31 kromofobnog karcinoma i 42 karcinoma svijetlih stanica bubrega s rubnim tkivom bubrega. Ispitivane molekule kadherinsko-kateninskog sustava statistički su pokazale značajno različite izraženosti između svih ispitivanih skupina čime smo potvrdili hipotezu istraživanja. Niti jedna od molekula kadherinskog sustava nije pokazala neovisnu pojedinačnu prognostičku vrijednost. Različiti, a dijelom i oprečni rezultati brojnih studija su dovoljno intrigantni te zahtijevaju nastavak istraživanja uloge i značaja ovih biljega u nastanku i progresiji tumora.

Abstract (english)

The recent studies have demonstrated that the expression of the cell adhesion molecules in some tumors is of the crucial importance in cancer invasion. The hypothesis of this study was that the oncocytomas and renal cell carcinomas (chromophobe and clear cell type) have different immunohistochemical expression of E-cadherin, N-cadherin and beta-catenin. The objective of this research was to determinate and compare immunohistochemical expression of E-cadherin, N-cadherin and beta-cathenin in kidney tissues, oncocytomas and renal cell carcinomas (chromophobe and clear cell type). In the research we used the archival paraffin blocks of 29 oncocytomas, 31 chromophobe and 42 clear cell renal carcinomas with non-neoplastic peripheral kidney tissue. All tested molecules of cadherin-catenin complex statistically showed significant differences among the groups but none of the molecules did not showed individual prognostic significance. Different and partly contrary results of many researches are interesting and still need to be clarified by additional research to find out role and meaning of these molecules in tumor’ s development and progression.

• Keywords: imunohistokemija; onkocitom; karcinom bubrega; E-kadherin; N-kadherin; beta-katenin
• Keywords (english): immunohistochemistry; oncocytoma; renal cell carcinoma; E-cadherin; N-cadherin; beta-catenin
• Language: croatian
• URN:NBN: urn:nbn:hr:217:325663
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Closed access
• Repository: Repository of the Faculty of Science
• Created on: 2017-05-19 12:40:04