Karakterizacija poliklonskih antitijela IgY iz kokošjih jaja za protein CA 15-3 te njihova specifičnost za homologni protein u serumu kuja

master's thesis

• Title (english): Characterisation of Polyclonal IgY Chicken Egg Antibodies for CA 15-3 Protein and Its Specificity for Homologous Protein in Bitch Serum
• Author: Leo Jakšić
• Mentor: Željko Gottstein (mentor)
• Mentor: Dubravko Pavoković (mentor)
• Committee member: Danijela Horvatek Tomić (predsjednik povjerenstva)
• Committee member: Dubravko Pavoković (član povjerenstva)
• Committee member: Željko Gottstein (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Poultry Diseases with Clinic); Zagreb
• Defense date and country: 2016-11-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

Neoplazije u pasa uzrokuju preko 50% uginuća u dobi iznad 10 godina, a najčešća u ženki pasa je neoplazija mliječne žlijezde, koja se može javiti već u dobi od 4 godine. Osim benignih, lokaliziranih oblika ove bolesti, u čak 80% slučajeva može se javiti u malignom obliku s metastazama po organizmu. Mogućnost pravovremene terapije u slučaju neoplazije mliječne žlijezde danas pridonosi izlječenju ili značajnom produženju života oboljelog psa kao i boljim uvjetima života, pa je rana dijagnostika iznimno važna. Tumorski marker iz seruma, CA 15-3, nedavno preuzet iz humane medicine gdje je korišten u dijagnostici raka dojke u žena, i u pasa se pokazao obečavajućim.
Cilj diplomskog rada je istražiti kvalitetu poliklonskih anti-CA 15-3 IgY antitijela iz kokošjih jaja i njihovu specifičnost za tumorski marker u serumu kuja.

Abstract (english)

Neoplasia in dogs cause over 50% deaths at the age over 10 years, and the most common form in female dogs is the mammary gland tumor, which can appear as soon as at 4 years of age. Aside from benign, localised forms of this disease, a malignant, metastatic form may appear in as much as 80% of cases. Application of therapy at the right time in case of a mammary gland neoplasia contributes to the healing or the distinct increase of life expectancy of a diseased dog, as well as contributes to a finer conditions of life, therefore early diagnosis is of extreme importance. A serum-extracted tumor marker, CA 15-3, which has recently been adopted from the fields of human medicine, has been used in breast cancer diagnostics, and has shown great promise in dogs as well.
The aim of this research is to investigate the quality of policlonal anti-CA 15-3 IgY antibodies extracted out of chiken eggs, and their specificity for the tumor marker in female dog serum.

• Keywords: IgY antitijela; karcinom mliječne žlijezde; CA 15-3 marker; PEG; elektroforeza; Western blot
• Keywords (english): IgY antibodies; mammary gland carcinoma; CA 15-3 marker; PEG electrophoresis; Western blot
• Language: croatian
• URN:NBN: urn:nbn:hr:178:031338
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Repository: Repository of Faculty of Veterinary Medicine
• Created on: 2018-07-23 10:36:09