Analiza srodnosti dinarske populacije euroazijskog risa (Lynx Lynx) pomoću mikrosatelitskih markera

master's thesis

• Title (english): Kinshipanalysis of Eurasian Lynx /Lynx lynx) Dinaric Populationusing Microsatellite Markers
• Author: Martina Đurčević
• Mentor: Tomislav Gomerčić (mentor)
• Mentor: Magda Sindičić (mentor)
• Committee member: Đuro Huber
• Committee member: Josip Kusak
• Committee member: Tomislav Gomerčić
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Biology); Zagreb
• Defense date and country: 2015-01-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Basic and Preclinical Veterinary Sciences

Abstract

Dinarska populacija euroazijskog risa (Lynx lynx) razvila se reintrodukcijom šest
jedinki koje su 1973. godine naseljene iz slovackih Karpata u Sloveniju, odakle se populacija
proširila u Hrvatsku te Bosnu i Hercegovinu. Danas se smatra da je u Hrvatskoj prisutno
najviše 40 do 60 jedinki, te da su uzroci ugroženosti populacije krivolov, nedostatak plijena i
niska genska raznolikost uzrokovana parenjem u srodstvu. Istraživanja su dokazala nisku
raznolikost alela mikrosatelitnih lokusa kod Dinarske populacije, te je cilj ovog rada bio
utvrditi koji od dostupnih kompjuterskih programa je najprikladniji za analizu srodnosti kod
Dinarske populacije risa. Analiza roditeljstva je metoda kojom se odreduje roditelj pojedinca
ili skupine pojedinaca koristeci genetske informacije kombinirane s razlicitim statistickim
metodama. U istraživanju sam koristila genotipove 19 mikrosatelitskih lokusa 91 jedinke
risova iz Dinarske populacije, te sam upotrebom tri racunalna programa analizirala
potencijalne roditelje za 29 životinja ciji uzorci su prikupljeni u razdoblju od 2000. do 2010.
godine. Utvrdila sam da su programi GIMLET i CERVUS zbog niske raznolikosti alela
neprikladni za upotrebu kod Dinarske populacije, dok su programom COLONY dobiveni
rezultati s najvecom pouzdanošcu, te sam na temelju tih rezultata uspjela izraditi precizno i
pouzdano obiteljsko stablo dinarske populacije risova.

Abstract (english)

Eurasian lynx (Lynx lynx) Dinaric population was founded in 1973 by reintroduction
of six animals from Slovak Carpathians to Slovenia, where from animals spread to Croatia
and Bosnia and Herzegovina. Today it is considered that lynx population in Croatia has a
maximum of 40 to 60 individuals. Population is threatened by poaching, lack of prey and low
genetic diversity due to inbreeding. The goal of this research was to investigate which
software is the most suitable for kinship analysis of the Dinaric lynx, as population has low
microsatellite variability. Kinship analysis determines parents of an individual or group of
individuals using genetic markers and different statistical methods. In this study I used
genotypes of 19 microsatellite loci of 91 individual lynx from Dinaric population. Using three
different programs I analyzed kinship for 29 individuals sampled in the 2000 – 2010 period. I
concluded that methods implemented in programs GIMLET and CERVUS are not suitable for
the kinship analysis of the Dinaric lynx population due to the low allelic diversity. Results
obtained with program COLONY had the highest reliability, so I managed to reconstruct
accurate and reliable pedigree of lynx population in Croatia.

• Keywords: euroazijski ris; Lynx lynx; mikrosateliti; srodnost; genska raznolikost
• Keywords (english): Eurasian lynx; Lynx lynx; microsatellites; kinship; genetic diversity
• Language: croatian
• URN:NBN: urn:nbn:hr:178:656447
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Repository: Repository of Faculty of Veterinary Medicine
• Created on: 2016-01-12 09:25:08