Utjecaj koncentracije otopine prekursora na biosintezu nanočestica željeza primjenom biljnog ekstrakta melise (Melissa officinalis) kao biokatalizatora

undergraduate thesis

• Title (english): Effect of precursor concentration on biosynthesis of Iron nanoparticles by application Melissa extract (Melissa officinalis) as biocatalyst
• Author: Marta Majetić
• Mentor: Maja Benković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2023-07-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Jedan od zadataka nanotehnologije je sinteza nanočestica, čestica u rasponu 1 – 100 nm. Kod postupka biosinteze, kao katalizatori se mogu koristiti biljke, gljive ili drugi biološki materijali. Cilj rada bio je ispitati utjecaj koncentracije prekursora, FeCl₃·6H₂O, na biosintezu nanočestica željeza. Korišteni biokatalizator je vodeni ekstrakt melise. Melisa je bogata fitokemikalijama, poput fenolnih tvari, koje se troše za provedbu biosinteze. To je dokazano mjerenjem ukupnih polifenola i antioksidacijskog kapaciteta u ekstraktu i otopinama prekursora. Svojstva smjesa i otopina okarakterizirana su mjerenjem boje, pH te električne vodljivosti. Sintetiziranim nanočesticama određeni su hidrodinamički promjer, boja i masa, dok su spektrofotometrijskim metodama identificirani karakteristični pikovi za otopine prekursora kao i reakcijske smjese. Dokazano je da je najviše sintetiziranih nanočestica iz prekursora najveće koncentracije (0,1 M) kao i da se povećanjem otopine prekursora troši više polifenola za biosintezu.

Abstract (english)

One of the main tasks in nanotechnology is the synthesis of nanoparticles, particles in the size range 1 – 100 nm. In biosynthesis, biocatalysts can be plants, fungi or other biological materials. The aim of this thesis was to investigate the effect of the precursor concentration, FeCl₃·6H₂O, on iron nanoparticle biosynthesis. An aqueous melissa extract was used as a biocatalyst. Melissa is rich in phytochemicals, such as phenolic compounds, which are necessary for the implementation of biosynthesis. That was proved by determining total phenolic content and antioxidant capacity. Properties of mixtures and solutions were characterized by measuring color, pH and electrical capacity. On synthesized nanoparticles were determined hydrodynamic diameter, color and mass, while using spectrophotometric methods were found characteristic peaks of solutions and mixtures. It was determined that with the highest precursor concentration (0,1 M) synthesized the most nanoparticles and with increasing precursor concentration, more polyphenols were used for biosynthesis.

• Keywords: biosinteza; nanočestice; željezo; Melissa officinalis
• Keywords (english): biosynthesis; nanoparticles; iron; Melissa officinalis
• Language: croatian
• URN:NBN: urn:nbn:hr:159:663802
• Study programme: Title: Food Technology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Repository: Repository of the Faculty of Food Technology and Biotechnology
• Created on: 2024-05-06 07:54:47