Praćenje antiagregacijske terapije metodom optičke agregometrije na analitičkom sustavu Sysmex CS-2500

master's thesis

• Title (english): Monitoring of antiplatelet therapy by optical aggregometry on Sysmex CS-2500 analytical system
• Author: Ivona Kuktić
• Mentor: Sandra Šupraha Goreta (mentor)
• Mentor: Sandra Margetić (mentor)
• Committee member: Sandra Šupraha Goreta (predsjednik povjerenstva)
• Committee member: Nada Vrkić (član povjerenstva)
• Committee member: Sandra Margetić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biochemistry and molecular biology); Zagreb
• Defense date and country: 2018-09-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Antiagregacijski (antitrombocitni) lijekovi posljednih se godina sve češće koriste u prevenciji i liječenju arterijske tromboze koja se klinički očituje akutnim ishemijskim dogaĎajima. Kontinuirani porast primjene ovih lijekova nametnuo je potrebu za uvoĎenjem agregometrije trombocita u procjeni učinkovitosti liječenja. Iako ove pretrage još uvijek nisu sastavni dio rutinske laboratorijske dijagnostike zbog nedovoljne standardizacije, očita je potreba za njezinim uvoĎenjem u praćenju liječenja ovih bolesnika. Cilj ovog rada bio je ispitati utjecaj antiagregacijskih lijekova na agregabilnost trombocita uz primjenu specifičnih agonista za pojedinu skupinu lijekova (acetilsalicilna kiselina (ASA) i/ili inhibitori ADP receptora) metodom optičke agregometrije na analitičkom sustavu Sysmex CS-2500. Agregabilnost trombocita bila je značajno snižena za obe skupine antiagregacijskih lijekova u odnosu na referentne vrijednosti. U bolesnika kod kojih je antiagregacijska terapija ukinuta zbog operativnog zahvata, oporavak funkcije trombocita postiže se brže u bolesnika liječenih ASA-om, u odnosu na bolesnike liječene inhibitorom ADP receptora ili kombiniranom terapijom.

Abstract (english)

In recent years inhibitors of platelet aggregation (antiplatelet drugs) are increasingly used in the prevention and treatment of arterial thrombosis that is clinically manifested by acute ischemic events. Continued increase in the use of these drugs has imposed the need for introducing platelet aggregometry to assess the efficiency of treatment. Although these assays still are not a part of routine laboratory diagnostics due to insufficient standardization, the need for its introduction in treatment of these patients is apparent. The aim of this study was to investigate the effect of antiplatelet drugs on platelet aggregation using specific agonists for particular drug group (acetylsalicylic acid (ASA) and /or ADP receptor inhibitors) by light transmission aggregometry method on the Sysmex CS 2500 analytical system. Platelet aggregation was significantly reduced for both groups of antiplatelet drugs compared to the reference values. In patients with antiplatelet therapy abolished due to surgery, recovery of platelet function was achieved faster in patients treated with ASA compared to patients treated with inhibitors of ADP receptors or those with combined therapy.

• Keywords: primarna hemostaza; trombociti; optička agregometrija; arterijska tromboza; antiagregacijska terapija; acetilsalicilna kiselina; inhibitori ADP receptora
• Keywords (english): platelet; platelet aggregation inhibitors; the optical light transmission aggregometry; primary hemostasis; arterial thrombosis; acetylsalicylic acid; ADP receptor inhibitors
• Language: croatian
• URN:NBN: urn:nbn:hr:163:364540
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb
• Created on: 2019-04-12 09:44:48