It is well known that microelements are essential for normal growth and development of the plant, and their additional input into the soil directly affects on the yield and quality of the plants. Microelements such as zinc, manganese, copper and iron are involved in various biological processes in the plant, and are of particular importance in the metabolic pathways, synthesis of various bioactive compounds. The aim of this study was to determine the effect of fertilizer with different composition of microelements on pomological characteristics and content of bioactive compounds in strawberry fruit. The plants treated with microelement fertilizers achieved significantly higher values in weight and size of the fruit, respectively, significant effect on the morphological properties of treated plants was determined. In addition to the morphological properties of the fruit, the application of microelement fertilizers also had a significant effect on the content of certain physicochemical properties of the raw material, namely on the content of: total dry matter, total soluble solids and total acids, and some bioactive compounds such as vitamin C and anthocyanins. All mentioned parameters have been found to be significantly higher when treated by microelement fertilizer. Microelement fertilization treatment negatively affected on the fruit firmness. Also, no significant differences in the color of the fruit between the control group and the treated plants were not determined i.e. the treatment with fertilizer did not significantly affect on the change of strawberry color. Based on the obtained results can be concluded that using microelement based fertilizers can affect the morphological properties and some bioactive compounds directly related to the quality of strawberry fruit.